GEPHE SUMMARY
Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000435
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Arabidopsis thaliana- Col0
Trait State in Taxon B
Arabidopsis thaliana - Kashmir
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Latin Name
Common Name
thale cress
Synonyms
thale cress; mouse-ear cress; thale-cress; Arabidopsis thaliana (L.) Heynh.; Arabidopsis thaliana (thale cress); Arabidopsis_thaliana; Arbisopsis thaliana; thale kress
Rank
species
Lineage
Show more ...
; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Arabidopsis thaliana- Col0
Taxon B
Latin Name
Common Name
thale cress
Synonyms
thale cress; mouse-ear cress; thale-cress; Arabidopsis thaliana (L.) Heynh.; Arabidopsis thaliana (thale cress); Arabidopsis_thaliana; Arbisopsis thaliana; thale kress
Rank
species
Lineage
Show more ...
; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Arabidopsis thaliana - Kashmir
GENOTYPIC CHANGE
Generic Gene Name
HAC1
Synonyms
ARABIDOPSIS HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 1; ARABIDOPSIS THALIANA P300/CBP ACETYLTRANSFERASE-RELATED PROTEIN 2; ATHAC1; ATHPCAT2; histone acetyltransferase of the CBP family 1; P300/CBP ACETYLTRANSFERASE-RELATED PROTEIN 2; PCAT2; YUP8H12R.38; YUP8H12R_38; At1g79000; YUP8H12R_22
String
Sequence Similarities
-
GO - Molecular Function
GO:0008270 : zinc ion binding
... show more
GO - Biological Process
GO - Cellular Component
UniProtKB
Arabidopsis thaliana
Arabidopsis thaliana
Presumptive Null
Molecular Type
Aberration Type
Deletion Size
1-9 bp
Molecular Details of the Mutation
1bp deletion resulting in frameshift
Experimental Evidence
Main Reference
Authors
Chao DY; Chen Y; Chen J; Shi S; Chen Z; Wang C; Danku JM; Zhao FJ; et al.
... show more
Abstract
Inorganic arsenic is a carcinogen, and its ingestion through foods such as rice presents a significant risk to human health. Plants chemically reduce arsenate to arsenite. Using genome-wide association (GWA) mapping of loci controlling natural variation in arsenic accumulation in Arabidopsis thaliana allowed us to identify the arsenate reductase required for this reduction, which we named High Arsenic Content 1 (HAC1). Complementation verified the identity of HAC1, and expression in Escherichia coli lacking a functional arsenate reductase confirmed the arsenate reductase activity of HAC1. The HAC1 protein accumulates in the epidermis, the outer cell layer of the root, and also in the pericycle cells surrounding the central vascular tissue. Plants lacking HAC1 lose their ability to efflux arsenite from roots, leading to both increased transport of arsenic into the central vascular tissue and on into the shoot. HAC1 therefore functions to reduce arsenate to arsenite in the outer cell layer of the root, facilitating efflux of arsenic as arsenite back into the soil to limit both its accumulation in the root and transport to the shoot. Arsenate reduction by HAC1 in the pericycle may play a role in limiting arsenic loading into the xylem. Loss of HAC1-encoded arsenic reduction leads to a significant increase in arsenic accumulation in shoots, causing an increased sensitivity to arsenate toxicity. We also confirmed the previous observation that the ACR2 arsenate reductase in A. thaliana plays no detectable role in arsenic metabolism. Furthermore, ACR2 does not interact epistatically with HAC1, since arsenic metabolism in the acr2 hac1 double mutant is disrupted in an identical manner to that described for the hac1 single mutant. Our identification of HAC1 and its associated natural variation provides an important new resource for the development of low arsenic-containing food such as rice.
Additional References
EXTERNAL LINKS
COMMENTS
Mapped independentyl in two studies
YOUR FEEDBACK is welcome!