GEPHE SUMMARY
Entry Status
Published
GepheID
GP00000446
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Thlaspi caerulescens - Ganges; Cd-accumulating
Trait State in Taxon B
Thlaspi caerulescens - Prayon
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Thlaspi caerulescens; Noccaea caerulescens (J.Presl & C.Presl) F.K.Mey., 1973; Thlaspi caerulescens J.Presl & C.Presl, 1819; Noccaeae caerulescens
Rank
species
Lineage
Show more ... ; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Coluteocarpeae; Noccaea
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Thlaspi caerulescens; Noccaea caerulescens (J.Presl & C.Presl) F.K.Mey., 1973; Thlaspi caerulescens J.Presl & C.Presl, 1819; Noccaeae caerulescens
Rank
species
Lineage
Show more ... ; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Coluteocarpeae; Noccaea
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Thlaspi caerulescens - Prayon
GENOTYPIC CHANGE
Generic Gene Name
HMA3
Synonyms
-
String
-
Sequence Similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
UniProtKB
Arabidopsis thaliana
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
Copy number Variant
Experimental Evidence
Authors
Ueno D; Milner MJ; Yamaji N; Yokosho K; Koyama E; Clemencia Zambrano M; Kaskie M; Ebbs S; et al. ... show more
Abstract
Cadmium (Cd) is a highly toxic heavy metal for plants, but several unique Cd-hyperaccumulating plant species are able to accumulate this metal to extraordinary concentrations in the aboveground tissues without showing any toxic symptoms. However, the molecular mechanisms underlying this hypertolerance to Cd are poorly understood. Here we have isolated and functionally characterized an allelic gene, TcHMA3 (heavy metal ATPase 3) from two ecotypes (Ganges and Prayon) of Thlaspi caerulescens contrasting in Cd accumulation and tolerance. The TcHMA3 alleles from the higher (Ganges) and lower Cd-accumulating ecotype (Prayon) share 97.8% identity, and encode a P(1B)-type ATPase. There were no differences in the expression pattern, cell-specificity of protein localization and transport substrate-specificity of TcHMA3 between the two ecotypes. Both alleles were characterized by constitutive expression in the shoot and root, a tonoplast localization of the protein in all leaf cells and specific transport activity for Cd. The only difference between the two ecotypes was the expression level of TcHMA3: Ganges showed a sevenfold higher expression than Prayon, partly caused by a higher copy number. Furthermore, the expression level and localization of TcHMA3 were different from AtHMA3 expression in Arabidopsis. Overexpression of TcHMA3 in Arabidopsis significantly enhanced tolerance to Cd and slightly increased tolerance to Zn, but did not change Co or Pb tolerance. These results indicate that TcHMA3 is a tonoplast-localized transporter highly specific for Cd, which is responsible for sequestration of Cd into the leaf vacuoles, and that a higher expression of this gene is required for Cd hypertolerance in the Cd-hyperaccumulating ecotype of T. caerulescens.

© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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