GEPHE SUMMARY
Entry Status
Published
GepheID
GP00000045
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Bactrocera dorsalis- sensitive
Trait State in Taxon B
Bactrocera dorsalis- artificially selected for resistance
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
oriental fruit fly
Synonyms
Bactrocera (Bactrocera) dorsalis; Bactrocera (Bactrocera) invadens; Bactrocera invadens; Bactrocera papayae; Bactrocera philippinensis; oriental fruit fly; Philippines fruit fly; papaya fruit fly; Bactrocera dorsalis (Hendel, 1912); Bactrocera invadens Drew, Tsuruta & White, 2005; Bactrocera philippinensis Drew & Hancock, 1994
Rank
species
Lineage
Show more ... ra; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Tephritoidea; Tephritidae; Dacinae; Dacini; Bactrocera; Bactrocera; Bactrocera dorsalis complex
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
oriental fruit fly
Synonyms
Bactrocera (Bactrocera) dorsalis; Bactrocera (Bactrocera) invadens; Bactrocera invadens; Bactrocera papayae; Bactrocera philippinensis; oriental fruit fly; Philippines fruit fly; papaya fruit fly; Bactrocera dorsalis (Hendel, 1912); Bactrocera invadens Drew, Tsuruta & White, 2005; Bactrocera philippinensis Drew & Hancock, 1994
Rank
species
Lineage
Show more ... ra; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae; Tephritoidea; Tephritidae; Dacinae; Dacini; Bactrocera; Bactrocera; Bactrocera dorsalis complex
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Mutation #1
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
I214V + G488S (+ possibly Q643R)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Ile Val 214
Authors
Hsu JC; Haymer DS; Wu WJ; Feng HT
Abstract
Mutations in the gene encoding the enzyme acetylcholinesterase (AChE) of the oriental fruit fly, Bactrocera dorsalis, associated with resistance to an organophosphorus insecticide have been characterized. Three point mutations producing nonsynonymous changes in the predicted amino acid sequence of the product of the B. dorsalis ace gene in resistant vs. susceptible flies have been identified. One of these changes is unique to B. dorsalis while the other two occur at sites that are identical to mutations previously described for another Bactrocera species. Although the precise role of the third mutation is not clearly established, the independent origin of two identical alterations in these two species strongly supports the idea proposed previously that molecular changes associated with insecticide resistance in key genes and enzymes such as AChE are largely constrained to a limited number of sites. The results obtained here also suggest that the widespread use of organophosphorus insecticides will likely lead to a predictable acquisition of resistance in wild populations of B. dorsalis as well as other pest species. For surveys of B. dorsalis populations that may develop resistance, diagnostic tests using PCR-RFLP based methods for detecting the presence of all three mutations in individual flies are described.
Additional References
Mutation #2
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
I214V + G488S (+ possibly Q643R)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid Gly Ser 488
Authors
Hsu JC; Haymer DS; Wu WJ; Feng HT
Abstract
Mutations in the gene encoding the enzyme acetylcholinesterase (AChE) of the oriental fruit fly, Bactrocera dorsalis, associated with resistance to an organophosphorus insecticide have been characterized. Three point mutations producing nonsynonymous changes in the predicted amino acid sequence of the product of the B. dorsalis ace gene in resistant vs. susceptible flies have been identified. One of these changes is unique to B. dorsalis while the other two occur at sites that are identical to mutations previously described for another Bactrocera species. Although the precise role of the third mutation is not clearly established, the independent origin of two identical alterations in these two species strongly supports the idea proposed previously that molecular changes associated with insecticide resistance in key genes and enzymes such as AChE are largely constrained to a limited number of sites. The results obtained here also suggest that the widespread use of organophosphorus insecticides will likely lead to a predictable acquisition of resistance in wild populations of B. dorsalis as well as other pest species. For surveys of B. dorsalis populations that may develop resistance, diagnostic tests using PCR-RFLP based methods for detecting the presence of all three mutations in individual flies are described.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
COMMENTS
The I214V substitution observed here in B. dorsalis is identical to one of the changes reported in the altered AChE enzyme described for a strain of B. oleae exhibiting high levels of organophosphate resistance (Vontas et al., 2002). This change is also equivalent to the I199V substitution in Drosophila (Mutero et al., 1994). The G488S substitution seen in B. dorsalis is also identical to a second change in the AChE enzyme structure in resistant B. oleae flies (Vontas et al., 2002). This substitution (G488) is also equivalent to the G396 in torpedo, or G474 in Drosophila.
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