GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000549
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Trifolium repens - cyanogenic
Trait State in Taxon B
Trifolium repens - acyanogenic
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
white clover
Synonyms
white clover; creeping white clover; Trifolium repens L.; Triflorum repens
Rank
species
Lineage
Show more ... ophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade; Trifolieae; Trifolium
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
white clover
Synonyms
white clover; creeping white clover; Trifolium repens L.; Triflorum repens
Rank
species
Lineage
Show more ... ophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade; Trifolieae; Trifolium
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
LI
Synonyms
-
String
-
Sequence Similarities
Belongs to the glycosyl hydrolase 1 family.
GO - Cellular Component
-
UniProtKB
Trifolium repens
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Deletion Size
unknown
Molecular Details of the Mutation
Gene deletion
Experimental Evidence
Authors
Olsen KM; Hsu SC; Small LL
Abstract
White clover is polymorphic for cyanogenesis, with both cyanogenic and acyanogenic plants occurring in nature. This chemical defense polymorphism is one of the longest-studied and best-documented examples of an adaptive polymorphism in plants. It is controlled by two independently segregating genes: Ac/ac controls the presence/absence of cyanogenic glucosides; and Li/li controls the presence/absence of their hydrolyzing enzyme, linamarase. Whereas Li is well characterized at the molecular level, Ac has remained unidentified. Here we report evidence that Ac corresponds to a gene encoding a cytochrome P450 of the CYP79D protein subfamily (CYP79D15), and we describe the apparent molecular basis of the Ac/ac polymorphism. CYP79D orthologs catalyze the first step in cyanogenic glucoside biosynthesis in other cyanogenic plant species. In white clover, Southern hybridizations indicate that CYP79D15 occurs as a single-copy gene in cyanogenic plants but is absent from the genomes of ac plants. Gene-expression analyses by RT-PCR corroborate this finding. This apparent molecular basis of the Ac/ac polymorphism parallels our previous findings for the Li/li polymorphism, which also arises through the presence/absence of a single-copy gene. The nature of these polymorphisms may reflect white clover's evolutionary origin as an allotetraploid derived from cyanogenic and acyanogenic diploid progenitors.
RELATED GEPHE
Related Genes
Related Haplotypes
No matches found.
COMMENTS
YOUR FEEDBACK is welcome!