GEPHE SUMMARY
Entry Status
Published
GepheID
GP00000555
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Zea mays
Trait State in Taxon B
Zea mays - Southern corn leaf blight resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
Zea mays var. japonica; maize; Zea mays L.; Zea mays mays
Rank
species
Lineage
Show more ... phyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
Zea mays var. japonica; maize; Zea mays L.; Zea mays mays
Rank
species
Lineage
Show more ... phyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
LHT1
Synonyms
K1B16.3; K1B16_3; LYSINE AND HISTIDINE SPECIFIC TRANSPORTER; lysine histidine transporter 1; At5g40780
Sequence Similarities
Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
UniProtKB
Arabidopsis thaliana
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Insertion Size
100-999 bp
Molecular Details of the Mutation
354bp insertioin resulting in premature stop codon
Experimental Evidence
Authors
Zhao Y; Lu X; Liu C; Guan H; Zhang M; Li Z; Cai H; Lai J
Abstract
rhm1 is a major recessive disease resistance locus for Southern corn leaf blight (SCLB). To further narrow down its genetic position, F(2) population and BC(1) F(1) population derived from the cross between resistant (H95(rhm) ) and susceptible parents (H95) of maize (Zea mays) were constructed. Using newly developed markers, rhm1 was initially delimited within an interval of 2.5 Mb, and then finally mapped to a 8.56 kb interval between InDel marker IDP961-503 and simple sequence repeat (SSR) marker A194149-1. Three polymorphic markers IDP961-504, IDP B2-3 and A194149-2 were shown to be co-segregated with the rhm1 locus. Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 (LHT1). Comparative sequence analysis indicated that the LHT1 in H95(rhm) harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73, H95 and Mo17. The 354 bp insertion resulted in a truncation of the predicted protein of candidate resistance allele (LHT1-H95(rhm) ). Our results strongly suggest LHT1 as the candidate gene for rhm1 against SCLB. The tightly linked molecular markers developed in this study can be directly used for molecular breeding of resistance to Southern corn leaf blight in maize.

© 2012 Institute of Botany, Chinese Academy of Sciences.
Additional References
COMMENTS
YOUR FEEDBACK is welcome!