GEPHE SUMMARY Print
Gephebase Gene
MLO
Entry Status
Published
GepheID
GP00000672
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Hordeum vulgare - susceptible
Trait State in Taxon B
Hordeum vulgare - Ethiopian origin resistant cultivars
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
barley; Hordeum vulgare L.; Horedum vulgare
Rank
species
Lineage
Show more ... yta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
barley; Hordeum vulgare L.; Horedum vulgare
Rank
species
Lineage
Show more ... yta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Hordeum vulgare - Ethiopian origin resistant cultivars
GENOTYPIC CHANGE
Generic Gene Name
MLO
Synonyms
-
String
-
Sequence Similarities
Belongs to the MLO family.
UniProtKB
Hordeum vulgare
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Insertion Size
1-10 kb
Molecular Details of the Mutation
Tandem array duplication including promoter and partial CDS; resulting in loss-of-function by transcriptional interference
Experimental Evidence
Authors
Piffanelli P; Ramsay L; Waugh R; Benabdelmouna A; D'Hont A; Hollricher K; Jørgensen JH; Schulze-Lefert P; et al. ... show more
Abstract
Barley (Hordeum vulgare) has played a pivotal role in Old World agriculture since its domestication about 10,000 yr ago. Barley plants carrying loss-of-function alleles (mlo) of the Mlo locus are resistant against all known isolates of the widespread powdery mildew fungus. The sole mlo resistance allele recovered so far from a natural habitat, mlo-11, was originally retrieved from Ethiopian landraces and nowadays controls mildew resistance in the majority of cultivated European spring barley elite varieties. Here we use haplotype analysis to show that the mlo-11 allele probably arose once after barley domestication. Resistance in mlo-11 plants is linked to a complex tandem repeat array inserted upstream of the wild-type gene. The repeat units consist of a truncated Mlo gene comprising 3.5 kilobases (kb) of 5'-regulatory sequence plus 1.1 kb of coding sequence. These generate aberrant transcripts that impair the accumulation of both Mlo wild-type transcript and protein. We exploited the meiotic instability of mlo-11 resistance and recovered susceptible revertants in which restoration of Mlo function was accompanied by excision of the repeat array. We infer cis-dependent perturbation of transcription machinery assembly by transcriptional interference in mlo-11 plants as a likely mechanism leading to disease resistance.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
No matches found.
COMMENTS
YOUR FEEDBACK is welcome!