GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000674
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Solanum lycopersicum
Trait State in Taxon B
Solanum lycopersicum
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
tomato
Synonyms
Lycopersicon esculentum var. esculentum; Solanum esculentum; Solanum lycopersicum var. humboldtii; tomato; Lycopersicon esculentum Mill.; Solanum esculentum Dunal; Solanum lycopersicum L.; Lycopersicon lycopersicum; Lycopersicum esculentum; Solanum lycopersicon
Rank
species
Lineage
Show more ... heophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum; Lycopersicon
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
tomato
Synonyms
Lycopersicon esculentum var. esculentum; Solanum esculentum; Solanum lycopersicum var. humboldtii; tomato; Lycopersicon esculentum Mill.; Solanum esculentum Dunal; Solanum lycopersicum L.; Lycopersicon lycopersicum; Lycopersicum esculentum; Solanum lycopersicon
Rank
species
Lineage
Show more ... heophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum; Lycopersicon
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
MLO
Synonyms
-
String
-
Sequence Similarities
Belongs to the MLO family.
UniProtKB
Hordeum vulgare
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Deletion Size
10-99 bp
Molecular Details of the Mutation
19bp deletion resulting in frameshift
Experimental Evidence
Authors
Bai Y; Pavan S; Zheng Z; Zappel NF; Reinstädler A; Lotti C; De Giovanni C; Ricciardi L; et al. ... show more
Abstract
The resistant cherry tomato (Solanum lycopersicum var. cerasiforme) line LC-95, derived from an accession collected in Ecuador, harbors a natural allele (ol-2) that confers broad-spectrum and recessively inherited resistance to powdery mildew (Oidium neolycopersici). As both the genetic and phytopathological characteristics of ol-2-mediated resistance are reminiscent of powdery mildew immunity conferred by loss-of-function mlo alleles in barley and Arabidopsis, we initiated a candidate-gene approach to clone Ol-2. A tomato Mlo gene (SlMlo1) with high sequence-relatedness to barley Mlo and Arabidopsis AtMLO2 mapped to the chromosomal region harboring the Ol-2 locus. Complementation experiments using transgenic tomato lines as well as virus-induced gene silencing assays suggested that loss of SlMlo1 function is responsible for powdery mildew resistance conferred by ol-2. In progeny of a cross between a resistant line bearing ol-2 and the susceptible tomato cultivar Moneymaker, a 19-bp deletion disrupting the SlMlo1 coding region cosegregated with resistance. This polymorphism results in a frameshift and, thus, a truncated nonfunctional SlMlo1 protein. Our findings reveal the second example of a natural mlo mutant that possibly arose post-domestication, suggesting that natural mlo alleles might be evolutionarily short-lived due to fitness costs related to loss of mlo function.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
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