GEPHE SUMMARY Print
Entry Status
Published
GepheID
GP00000676
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Arabidopsis thaliana- Col0
Trait State in Taxon B
Arabidopsis thaliana- Ler0
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
thale cress
Synonyms
thale cress; mouse-ear cress; thale-cress; Arabidopsis thaliana (L.) Heynh.; Arabidopsis thaliana (thale cress); Arabidopsis_thaliana; Arbisopsis thaliana; thale kress
Rank
species
Lineage
Show more ... ; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Arabidopsis thaliana- Col0
Taxon B
Common Name
thale cress
Synonyms
thale cress; mouse-ear cress; thale-cress; Arabidopsis thaliana (L.) Heynh.; Arabidopsis thaliana (thale cress); Arabidopsis_thaliana; Arbisopsis thaliana; thale kress
Rank
species
Lineage
Show more ... ; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Arabidopsis thaliana- Ler0
GENOTYPIC CHANGE
Generic Gene Name
MOT1
Synonyms
F3N11.13; F3N11_13; molybdate transporter 1; ST5.2; SULTR5.2; At2g25680
Sequence Similarities
Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
UniProtKB
Arabidopsis thaliana
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Deletion Size
10-99 bp
Molecular Details of the Mutation
53bp deletion in promoter
Experimental Evidence
Authors
Baxter I; Muthukumar B; Park HC; Buchner P; Lahner B; Danku J; Zhao K; Lee J; et al. ... show more
Abstract
Molybdenum (Mo) is an essential micronutrient for plants, serving as a cofactor for enzymes involved in nitrate assimilation, sulfite detoxification, abscisic acid biosynthesis, and purine degradation. Here we show that natural variation in shoot Mo content across 92 Arabidopsis thaliana accessions is controlled by variation in a mitochondrially localized transporter (Molybdenum Transporter 1 - MOT1) that belongs to the sulfate transporter superfamily. A deletion in the MOT1 promoter is strongly associated with low shoot Mo, occurring in seven of the accessions with the lowest shoot content of Mo. Consistent with the low Mo phenotype, MOT1 expression in low Mo accessions is reduced. Reciprocal grafting experiments demonstrate that the roots of Ler-0 are responsible for the low Mo accumulation in shoot, and GUS localization demonstrates that MOT1 is expressed strongly in the roots. MOT1 contains an N-terminal mitochondrial targeting sequence and expression of MOT1 tagged with GFP in protoplasts and transgenic plants, establishing the mitochondrial localization of this protein. Furthermore, expression of MOT1 specifically enhances Mo accumulation in yeast by 5-fold, consistent with MOT1 functioning as a molybdate transporter. This work provides the first molecular insight into the processes that regulate Mo accumulation in plants and shows that novel loci can be detected by association mapping.
COMMENTS
Genetic heterogeneity; Complex locus with additional loci with multiple alleles; two promoter variants + 1 25kb downstream + LD with other site 600kb away with putative role of Copper Transporter 6 (COPT6)
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