GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000737
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Lactuca serriola accession UC96US23
Trait State in Taxon B
Lactuca sativa cv. Salinas
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Lactuca scariola; compass-plant; prickly lettuce; Lactuca serriola L.; Lactuca seriola
Rank
species
Lineage
Show more ... ta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae; Lactuca
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Lactuca serriola accession UC96US23
Taxon B
Latin Name
Common Name
-
Synonyms
cultivated lettuce; garden lettuce; Lactuca sativa L.; Lativa satuca
Rank
species
Lineage
Show more ... ta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae; Lactuca
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Lactuca sativa cv. Salinas
GENOTYPIC CHANGE
Generic Gene Name
CCD4
Synonyms
carotenoid cleavage dioxygenase 4; CCD4; nine-cis-epoxycarotenoid dioxygenase 4; T18B16.140; T18B16_140; NCED4; At4g19170
Sequence Similarities
Belongs to the carotenoid oxygenase family.
UniProtKB
Arabidopsis thaliana
GenebankID or UniProtKB
Presumptive Null
Molecular Type
Aberration Type
Molecular Details of the Mutation
unknown
Experimental Evidence
Authors
Argyris J; Truco MJ; Ochoa O; McHale L; Dahal P; Van Deynze A; Michelmore RW; Bradford KJ
Abstract
Thermoinhibition, or failure of seeds to germinate when imbibed at warm temperatures, can be a significant problem in lettuce (Lactuca sativa L.) production. The reliability of stand establishment would be improved by increasing the ability of lettuce seeds to germinate at high temperatures. Genes encoding germination- or dormancy-related proteins were mapped in a recombinant inbred line population derived from a cross between L. sativa cv. Salinas and L. serriola accession UC96US23. This revealed several candidate genes that are located in the genomic regions containing quantitative trait loci (QTLs) associated with temperature and light requirements for germination. In particular, LsNCED4, a temperature-regulated gene in the biosynthetic pathway for abscisic acid (ABA), a germination inhibitor, mapped to the center of a previously detected QTL for high temperature germination (Htg6.1) from UC96US23. Three sets of sister BC(3)S(2) near-isogenic lines (NILs) that were homozygous for the UC96US23 allele of LsNCED4 at Htg6.1 were developed by backcrossing to cv. Salinas and marker-assisted selection followed by selfing. The maximum temperature for germination of NIL seed lots with the UC96US23 allele at LsNCED4 was increased by 2-3°C when compared with sister NIL seed lots lacking the introgression. In addition, the expression of LsNCED4 was two- to threefold lower in the former NIL lines as compared to expression in the latter. Together, these data strongly implicate LsNCED4 as the candidate gene responsible for the Htg6.1 phenotype and indicate that decreased ABA biosynthesis at high imbibition temperatures is a major factor responsible for the increased germination thermotolerance of UC96US23 seeds.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
Functional evidence in mutant and RNAi lines
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