GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000753
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Zea mays - hard translucent endosperm
Trait State in Taxon B
Zea mays - soft opaque endosperm with lysine contents - Crow
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
-
Synonyms
Zea mays var. japonica; maize; Zea mays L.; Zea mays mays
Rank
species
Lineage
Show more ... phyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
-
Synonyms
Zea mays var. japonica; maize; Zea mays L.; Zea mays mays
Rank
species
Lineage
Show more ... phyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
O2
Synonyms
-
Sequence Similarities
Belongs to the bZIP family.
GO - Biological Process
-
GO - Cellular Component
UniProtKB
Zea mays
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Deletion Size
1-9 bp
Molecular Details of the Mutation
1bp deletion predicted to cause premature termination of translation
Experimental Evidence
Authors
Schmidt RJ; Burr FA; Burr B
Abstract
Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
1
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