GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000755
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Loligo forbesi (lambda max 494 nm)
Trait State in Taxon B
Alloteuthis subulata (lambda max 499 nm)
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Common Name
northern European squid
Synonyms
northern European squid; Loligo forbesii Steenstrup, 1856; Loligo forbesi
Rank
species
Lineage
Show more ... organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Neocoleoidea; Decapodiformes; Teuthida; Myopsina; Loliginidae; Loligo
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Loligo (Alloteuthis) subulata; Loligo subulata; Alloteuthis subulata (Lamarck, 1798)
Rank
species
Lineage
Show more ... nisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Neocoleoidea; Decapodiformes; Teuthida; Myopsina; Loliginidae; Alloteuthis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
F270S
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Morris A; Bowmaker JK; Hunt DM
Abstract
The molecular basis of spectral tuning of rhodopsin pigments in two squid species is examined. The absorbance spectra of rhodopsin extracts from Alloteuthis subulata (lambda max 499 nm) showed a 5 nm red shift compared with Loligo forbesi (lambda max 494 nm). The rhodopsin gene sequence of A. subulata opsin was determined from polymerase chain reaction (PCR) amplified fragments by using genomic DNA as template. The deduced amino acid sequence was compared with that obtained from the previously published cDNA sequence of Loligo forbesi. A total of 22 amino acid differences are present, although only seven can be considered to be non-homologous substitutions. Three of these changes occur in helical transmembrane regions but only one, the substitution of phenylalanine by serine at site 270 in Alloteuthis subulata, involves the replacement of an apolar with a hydroxyl-bearing residue at a site located near the centre of helix VI and on the inner face of the retinal-binding pocket. The equivalent site is also used for the spectral tuning of primate cone pigments, an example of convergent evolution. It is proposed that substitution at this site is responsible for the 5 nm red shift. The relation between this spectral shift and the maximum depth distribution of Alloteuthis subulata of about 200 m, compared with about 360 m for Loligo forbesi, is discussed. An unexpected finding was that the rhodopsin gene of both species appears to lack introns.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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