GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000828
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Culex pipiens
Trait State in Taxon B
Culex pipiens - resistant - allele 1
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
northern house mosquito
Synonyms
Culex agilis; Culex autogenicus; Culex azoriensis; Culex bicolor; Culex bifurcatus; Culex calcitrans; Culex calloti; Culex comitatus; Culex consobrinus; Culex dipseticus; Culex disjunctus; Culex doliorum; Culex domesticus; Culex erectus; Culex fasciatus; Culex haematophagus; Culex longefurcatus; Culex luteus; Culex marginalis; Culex melanorhinus; Culex meridionalis; Culex osakaensis; Culex pallipes; Culex phytophagus; Culex quasimodestus; Culex rufinus; Culex rufus; Culex sternopunctatus; Culex thoracicus; Culex torridus; Culex trifurcatus; Culex unistriatus; Culex varioannulatus; northern house mosquito; Culex pipiens Linnaeus, 1758
Rank
species
Lineage
Show more ... lata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Culicinae; Culicini; Culex; Culex; Culex pipiens complex
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
northern house mosquito
Synonyms
Culex agilis; Culex autogenicus; Culex azoriensis; Culex bicolor; Culex bifurcatus; Culex calcitrans; Culex calloti; Culex comitatus; Culex consobrinus; Culex dipseticus; Culex disjunctus; Culex doliorum; Culex domesticus; Culex erectus; Culex fasciatus; Culex haematophagus; Culex longefurcatus; Culex luteus; Culex marginalis; Culex melanorhinus; Culex meridionalis; Culex osakaensis; Culex pallipes; Culex phytophagus; Culex quasimodestus; Culex rufinus; Culex rufus; Culex sternopunctatus; Culex thoracicus; Culex torridus; Culex trifurcatus; Culex unistriatus; Culex varioannulatus; northern house mosquito; Culex pipiens Linnaeus, 1758
Rank
species
Lineage
Show more ... lata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Culicinae; Culicini; Culex; Culex; Culex pipiens complex
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
L1014F
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Chen L; Zhong D; Zhang D; Shi L; Zhou G; Gong M; Zhou H; Sun Y; et al. ... show more
Abstract
Pyrethroid insecticides have been extensively used in China and worldwide for public health pest control. Accurate resistance monitoring is essential to guide the rational use of insecticides and resistance management. Here we examined the nucleotide diversity of the para-sodium channel gene, which confers knockdown resistance (kdr) in Culex pipiens pallens mosquitoes in China. The sequence analysis of the para-sodium channel gene identified L1014F and L1014S mutations. We developed and validated allele-specific PCR and the real-time TaqMan methods for resistance diagnosis. The real-time TaqMan method is more superior to the allele-specific PCR method as evidenced by higher amplification rate and better sensitivity and specificity. Significant positive correlation between kdr allele frequency and bioassay-based resistance phenotype demonstrates that the frequency of L1014F and L1014S mutations in the kdr gene can be used as a molecular marker for deltamethrin resistance monitoring in natural Cx. pipiens pallens populations in the East China region. The laboratory selection experiment found that L1014F mutation frequency, but not L1014S mutation, responded to deltamethrin selection, suggesting that the L1014F mutation is the key mutation conferring resistance to deltamethrin. High L1014F mutation frequency detected in six populations of Cx. pipens pallens suggests high prevalence of pyrethroid resistance in Eastern China, calling for further surveys to map the resistance in China and for investigating alternative mosquito control strategies.
RELATED GEPHE
Related Haplotypes
1
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