GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000831
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Haematobia irritans
Trait State in Taxon B
Haematobia irritans - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
horn fly
Synonyms
Lyperosia irritans; horn fly; Haematobia irritans (Linnaeus, 1758); Haematobia iritans
Rank
species
Lineage
Show more ... oda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Calyptratae; Muscoidea; Muscidae; Muscinae; Stomoxyini; Haematobia
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
horn fly
Synonyms
Lyperosia irritans; horn fly; Haematobia irritans (Linnaeus, 1758); Haematobia iritans
Rank
species
Lineage
Show more ... oda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Calyptratae; Muscoidea; Muscidae; Muscinae; Stomoxyini; Haematobia
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
(=M918T)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Guerrero FD; Jamroz RC; Kammlah D; Kunz SE
Abstract
Two pyrethroid-resistant strains of horn flies were found to be 17- and 688-fold more resistant to permethrin and 17- and 11,300-fold more resistant to cyhalothrin than a susceptible control strain. Synergism experiments with piperonyl butoxide showed that both target site insensitivity and metabolic resistance mechanisms were present in the Super Resistant strain. Using the reverse transcriptase-polymerase chain reaction (RT-PCR), a 0.9 kb fragment of the putative sodium channel gene from susceptible and resistant flies was cloned and sequenced. Two sequence variants were detected, presumably arising from alternative splicing of transcripts. The amino acid sequences deduced from the resistant and susceptible fly gene fragments were identical except for three amino acid substitutions, two of which have been associated with resistance in house flies. A leucine to phenylalanine substitution associated with knockdown resistance (kdr) was found in both resistant strains. A methionine to threonine substitution associated with super-kdr was found in the Super Resistant strain. Translation of poly(A)+ RNA followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) detected translation products whose concentrations increased in association with pyrethroid resistance. Random-amplified polymorphic DNA (RAPD)-PCR of genomic DNA with over 260 DNA oligomers yielded one resistance-associated marker, designated HF-77, which was not detected in any susceptible flies but was present in 16% of the resistant individuals.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
1
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