GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000834
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Heliothis virescens
Trait State in Taxon B
Heliothis virescens - resistant - allele 2
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
tobacco budworm
Synonyms
tobacco budworm; Chloridea virescens; Helicoverpa virescens; Heliothis virescens (Fabricius, 1777)
Rank
species
Lineage
Show more ... poda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Heliothinae; Heliothis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
tobacco budworm
Synonyms
tobacco budworm; Chloridea virescens; Helicoverpa virescens; Heliothis virescens (Fabricius, 1777)
Rank
species
Lineage
Show more ... poda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Heliothinae; Heliothis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
V421M (=V410M)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Zhao Y; Park Y; Adams ME
Abstract
Pyrethroids are a class of voltage-dependent sodium channel modifiers widely used as insecticides for control of disease vectors and agricultural pests. Many insect populations have developed resistance to pyrethroids linked to nervous system insensitivity and structural mutations in neuronal sodium channels. Pyrethroid resistant strains of the moth Heliothis virescens carry single point mutations leading to amino acid substitutions in either transmembrane segment I-S6 (V421M) or II-S6 (L1029H) of the para-homologous sodium channel. We analyzed the consequences of V421M and L1029H mutations constructed in the Drosophila para sodium channel heterologously expressed in Xenopus oocytes, and found that both mutations confer channel insensitivity to permethrin, with the L1029H mutation having a more pronounced effect. Both mutations also modify the intrinsic voltage-dependent gating properties of the channel, but L1029H less so than V421M. These results suggest that mutation V421M exacts a higher fitness cost than L1029H, providing a plausible explanation for genetic succession observed in field strains, where V421M was replaced by L1029H during the past decade.

Copyright 2000 Academic Press.
RELATED GEPHE
Related Genes
Related Haplotypes
1
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