GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000840
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Hyalella azteca -sensitive to pyrethroids
Trait State in Taxon B
Hyalella azteca - resistant to pyrethroids
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
Hyalella azteca Saussure, 1858
Rank
species
Lineage
Show more ... sozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Peracarida; Amphipoda; Senticaudata; Talitrida; Talitroidea; Hyalellidae; Hyalella
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
Hyalella azteca Saussure, 1858
Rank
species
Lineage
Show more ... sozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Peracarida; Amphipoda; Senticaudata; Talitrida; Talitroidea; Hyalellidae; Hyalella
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
M918L
Experimental Evidence
Taxon A Taxon B Position
Codon ATG CTG -
Amino-acid Met Leu 918
Authors
Weston DP; Poynton HC; Wellborn GA; Lydy MJ; Blalock BJ; Sepulveda MS; Colbourne JK
Abstract
Use of pesticides can have substantial nonlethal impacts on nontarget species, including driving evolutionary change, often with unknown consequences for species, ecosystems, and society. Hyalella azteca, a species complex of North American freshwater amphipods, is widely used for toxicity testing of water and sediment and has frequently shown toxicity due to pyrethroid pesticides. We demonstrate that 10 populations, 3 from laboratory cultures and 7 from California water bodies, differed by at least 550-fold in sensitivity to pyrethroids. The populations sorted into four phylogenetic groups consistent with species-level divergence. By sequencing the primary pyrethroid target site, the voltage-gated sodium channel, we show that point mutations and their spread in natural populations were responsible for differences in pyrethroid sensitivity. At least one population had both mutant and WT alleles, suggesting ongoing evolution of resistance. Although nonresistant H. azteca were susceptible to the typical neurotoxic effects of pyrethroids, gene expression analysis suggests the mode of action in resistant H. azteca was not neurotoxicity but was oxidative stress sustained only at considerably higher pyrethroid concentrations. The finding that a nontarget aquatic species has acquired resistance to pesticides used only on terrestrial pests is troubling evidence of the impact of chronic pesticide transport from land-based applications into aquatic systems. Our findings have far-reaching implications for continued uncritical use of H. azteca as a principal species for monitoring and environmental policy decisions.
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
3
COMMENTS
@Parallelism - The same amico acid change has also been found in the same species, due to another nucleotide change
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