GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000858
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Other fishes
Trait State in Taxon B
Chionodraco hamatus
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Latin Name
Common Name
teleost fishes
Synonyms
teleost fishes
Rank
infraclass
Lineage
Show more ... ular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
Antarctic icefish
Synonyms
Chaenichthys rhinoceratus hamatus; Antarctic icefish; Chaenichthys rhinoceratus hamatus Lonnberg, 1905; Chionodraco hamatus (Lonnberg, 1905)
Rank
species
Lineage
Show more ... Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata; Acanthomorphata; Euacanthomorphacea; Percomorphaceae; Eupercaria; Perciformes; Notothenioidei; Channichthyidae; Chionodraco
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
slc15a1
Synonyms
-
String
-
Sequence Similarities
Belongs to the PTR2/POT transporter (TC 2.A.17) family.
UniProtKB
Chionodraco hamatus
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
C-terminal (cytosolic) de novo VDMSRKS domain conferring cold resistance
Experimental Evidence
Authors
Rizzello A; Romano A; Kottra G; Acierno R; Storelli C; Verri T; Daniel H; Maffia M
Abstract
Adaptation of organisms to extreme environments requires proteins to work at thermodynamically unfavorable conditions. To adapt to subzero temperatures, proteins increase the flexibility of parts of, or even the whole, 3D structure to compensate for the lower thermal kinetic energy available at low temperatures. This may be achieved through single-site amino acid substitutions in regions of the protein that undergo large movements during the catalytic cycle, such as in enzymes or transporter proteins. Other strategies of cold adaptation involving changes in the primary amino acid sequence have not been documented yet. In Antarctic icefish (Chionodraco hamatus) peptide transporter 1 (PEPT1), the first transporter cloned from a vertebrate living at subzero temperatures, we came upon a unique principle of cold adaptation. A de novo domain composed of one to six repeats of seven amino acids (VDMSRKS), placed as an extra stretch in the cytosolic COOH-terminal region, contributed per se to cold adaptation. VDMSRKS was in a protein region uninvolved in transport activity and, notably, when transferred to the COOH terminus of a warm-adapted (rabbit) PEPT1, it conferred cold adaptation to the receiving protein. Overall, we provide a paradigm for protein cold adaptation that relies on insertion of a unique domain that confers greater affinity and maximal transport rates at low temperatures. Due to its ability to transfer a thermal trait, the VDMSRKS domain represents a useful tool for future cell biology or biotechnological applications.
Additional References
RELATED GEPHE
Related Haplotypes
No matches found.
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