GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000862
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Glycine max; purple-blue flowers
Trait State in Taxon B
Glycine max; blue flowers
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Latin Name
Common Name
soybean
Synonyms
soybean; soybeans; Glycine max (L.) Merr.; Glycine max; cv. Wye
Rank
species
Lineage
Show more ... angiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
soybean
Synonyms
soybean; soybeans; Glycine max (L.) Merr.; Glycine max; cv. Wye
Rank
species
Lineage
Show more ... angiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
PH4
Synonyms
-
String
-
Sequence Similarities
-
GO - Biological Process
-
GO - Cellular Component
UniProtKB
Petunia hybrida
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsense
Molecular Details of the Mutation
Substitution creating premature Stop (codon 158)
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Takahashi R; Benitez ER; Oyoo ME; Khan NA; Komatsu S
Abstract
Previous studies revealed that the recessive allele of the W2 locus generated purple-blue color and high vacuolar pH of flower petals in soybean. The location of W2 gene was reportedly close to simple sequence repeat marker Satt318 in molecular linkage group B2. We used information from the soybean genome to clone a candidate gene for W2. An MYB transcription factor gene belonging to G20 group was found in the vicinity of Satt318. Full-length cDNAs were cloned from purple-flowered cultivar Harosoy (W2 allele) and purple-blue flowered cultivars, Nezumisaya and w2-20 (w2 allele), by reverse transcription-PCR and designated as GmMYB-G20-1. Its open reading frame was 1083 bp long that encoded 361 amino acids in Harosoy. GmMYB-G20-1 had 53.7% similarity in amino acid sequence with the PH4 gene of petunia controlling blueness and vacuolar pH of flower petals. GmMYB-G20-1 of Nezumisaya and w2-20 had 3 base substitutions compared with that of Harosoy. The first substitution generated a stop codon in the MYB domain, resulting in truncated polypeptides. Cleaved amplified polymorphic sequence (CAPS) marker was developed to detect the base substitution. The polymorphic CAPS marker co-segregated with alleles at the W2 locus in the F(2) population. These results suggest that GmMYB-G20-1 might correspond to the W2 gene.
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