GEPHE SUMMARY Print
Entry Status
Published
GepheID
GP00000884
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Oryza sativa - blast susceptible 99SL44
Trait State in Taxon B
Oryza sativa - blast resistant Tsuyuake
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Latin Name
Common Name
rice
Synonyms
rice; red rice; Oryza sativa L.
Rank
species
Lineage
Show more ... ytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Oryza sativa - blast susceptible 99SL44
Taxon B
Latin Name
Common Name
rice
Synonyms
rice; red rice; Oryza sativa L.
Rank
species
Lineage
Show more ... ytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Oryza sativa - blast resistant Tsuyuake
GENOTYPIC CHANGE
Generic Gene Name
PIKM1-TS
Synonyms
PI-KM1
String
-
Sequence Similarities
Belongs to the disease resistance NB-LRR family.
GO - Cellular Component
-
UniProtKB
Oryza sativa subsp. japonica
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
Molecular Details of the Mutation
Coding variation in both genes; with alleles of both genes necessary for resistance in completementation assays
Experimental Evidence
Authors
Ashikawa I; Hayashi N; Yamane H; Kanamori H; Wu J; Matsumoto T; Ono K; Yano M
Abstract
The rice blast resistance gene Pikm was cloned by a map-based cloning strategy. High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval. Sequence analysis predicted two adjacently arranged resistance-like genes, Pikm1-TS and Pikm2-TS, within this candidate region. These genes encoded proteins with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) and were considered the most probable candidates for Pikm. However, genetic complementation analysis of transgenic lines individually carrying these two genes negated the possibility that either Pikm1-TS or Pikm2-TS alone was Pikm. Instead, it was revealed that transgenic lines carrying both of these genes expressed blast resistance. The results of the complementation analysis and an evaluation of the resistance specificity of the transgenic lines to blast isolates demonstrated that Pikm-specific resistance is conferred by cooperation of Pikm1-TS and Pikm2-TS. Although these two genes are not homologous with each other, they both contain all the conserved motifs necessary for an NBS-LRR class gene to function independently as a resistance gene.
EXTERNAL LINKS
COMMENTS
Cluster of paralogous genes
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