GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000921
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
C. elegans - all other strains - sensitive
Trait State in Taxon B
C. elegans Hawaii - insensitive
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
-
Synonyms
roundworm; Rhabditis elegans; Caenorhabditis elegans (Maupas, 1900); Rhabditis elegans Maupas, 1900
Rank
species
Lineage
Show more ... s; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
-
Synonyms
roundworm; Rhabditis elegans; Caenorhabditis elegans (Maupas, 1900); Rhabditis elegans Maupas, 1900
Rank
species
Lineage
Show more ... s; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
C. elegans Hawaii - insensitive
GENOTYPIC CHANGE
Generic Gene Name
-
Synonyms
-
String
-
Sequence Similarities
-
GO - Molecular Function
-
GO - Biological Process
-
GO - Cellular Component
-
UniProtKB
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Deletion Size
1-9 bp
Molecular Details of the Mutation
1bp deletion resulting in stop codon
Experimental Evidence
Authors
Tijsterman M; Okihara KL; Thijssen K; Plasterk RH
Abstract
One of the remarkable aspects about RNA interference (RNAi) in Caenorhabditis elegans is that the trigger molecules, dsRNA, can be administered via the animal's food. We assayed whether this feature is a universal property of the species by testing numerous strains that have been isolated from different parts of the globe. We found that one isolate from Hawaii had a defect in RNAi that was specific to the germline and was a result of multiple mutations in a PAZ/PIWI domain-containing protein, which we named PPW-1. Deleting ppw-1 in the canonical C. elegans strain Bristol N2 makes it resistant to feeding of dsRNA directed against germline-expressed genes. PPW-1 belongs to the Argonaute family of proteins, which act in posttranscriptional gene silencing and development, and is homologous to the RNAi gene rde-1. Our data indicate that at least two members of this family are required for complete and effective RNAi in C. elegans.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
COMMENTS
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