GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000939
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait
Trait State in Taxon A
Brassica oleracea var botrytis
Trait State in Taxon B
Brassica oleracea var botrytis -Purple
Ancestral State
Data not curated
Taxonomic Status
Taxon A
Common Name
wild cabbage
Synonyms
wild cabbage; Brassica oleracea L., 1753
Rank
species
Lineage
Show more ... ina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
NCBI Taxonomy ID
is Taxon A an Infraspecies?
Yes
Taxon A Description
Brassica oleracea var botrytis
Taxon B
Common Name
-
Synonyms
Brassica oleracea subsp. botrytis; cauliflower; Brassica oleracea var. botrytis L.; Brassica oleracea botrytis
Rank
varietas
Lineage
Show more ... racheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica; Brassica oleracea
NCBI Taxonomy ID
is Taxon B an Infraspecies?
Yes
Taxon B Description
Brassica oleracea var botrytis -Purple
GENOTYPIC CHANGE
Generic Gene Name
MYB2
Synonyms
-
String
-
Sequence Similarities
-
GO - Biological Process
-
GO - Cellular Component
UniProtKB
Brassica oleracea var. botrytis
GenebankID or UniProtKB
Presumptive Null
Molecular Type
Aberration Type
Molecular Details of the Mutation
unknown
Experimental Evidence
Authors
Chiu LW; Zhou X; Burke S; Wu X; Prior RL; Li L
Abstract
Anthocyanins are responsible for the color of many flowers, fruits, and vegetables. An interesting and unique Purple (Pr) gene mutation in cauliflower (Brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a few other tissues. To unravel the nature of the Pr mutation in cauliflower, we isolated the Pr gene via a combination of candidate gene analysis and fine mapping. Pr encoded a R2R3 MYB transcription factor that exhibited tissue-specific expression, consistent with an abnormal anthocyanin accumulation pattern in the mutant. Transgenic Arabidopsis (Arabidopsis thaliana) and cauliflower plants expressing the Pr-D allele recapitulated the mutant phenotype, confirming the isolation of the Pr gene. Up-regulation of Pr specifically activated a basic helix-loop-helix transcription factor and a subset of anthocyanin structural genes encoding flavonoid 3'-hydroxylase, dihydroflavonol 4-reductase, and leucoanthocyanidin dioxygenase to confer ectopic accumulation of pigments in the purple cauliflower. Our results indicate that the genetic variation including a Harbinger DNA transposon insertion in the upstream regulatory region of the Pr-D allele is responsible for the up-regulation of the Pr gene in inducing phenotypic change in the plant. The successful isolation of Pr provides important information on the regulatory control of anthocyanin biosynthesis in Brassica vegetables, and offers a genetic resource for development of new varieties with enhanced health-promoting properties and visual appeal.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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