GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00000973
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Ctenocephalides felis
Trait State in Taxon B
Ctenocephalides felis - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
cat flea
Synonyms
cat flea; Ctenocephalides felis (Bouche, 1835)
Rank
species
Lineage
Show more ... rthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Siphonaptera; Pulicomorpha; Pulicoidea; Pulicidae; Archaeopsyllinae; Ctenocephalides
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
cat flea
Synonyms
cat flea; Ctenocephalides felis (Bouche, 1835)
Rank
species
Lineage
Show more ... rthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Siphonaptera; Pulicomorpha; Pulicoidea; Pulicidae; Archaeopsyllinae; Ctenocephalides
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
Rdl
Synonyms
CG10537; CT29555; Dmel\CG10537; DmRdl; DmRDL; gaba; GABA; GABA-R; GABAr; GABA[[A]]; GABA[[A]] receptor; GABA[[A]]-R; GABA[[A]]R; LCCH1; Rd1; rdl; RDL
Sequence Similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily.
UniProtKB
Drosophila melanogaster
GenebankID or UniProtKB
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
Ala302Ser
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Bass C; Schroeder I; Turberg A; Field LM; Williamson MS
Abstract
In many insect species, resistance to cyclodiene insecticides is caused by amino acid substitutions at a single residue (A302) within the M2 transmembrane region of the gamma-aminobutyric acid (GABA) receptor sub-unit termed Rdl (resistance to dieldrin). These mutations (A302S and A302G) have also been shown to confer varying levels of cross-resistance to fipronil, a phenylpyrazole insecticide with a similar mode of action to cyclodienes. To investigate the possible occurrence of these mutations in the cat flea, Ctenocephalides felis (Bouché), a 176-bp fragment of the cat flea Rdl gene, encompassing the mutation site, was PCR amplified and sequenced from nine laboratory flea strains. The A302S mutation was found in eight of the nine strains analysed, although the relative frequency of the mutant allele varied between strains. Only one strain (R6) was found to be homozygous for the S302 allele in all the individuals tested, and this correlated with previous reports of low-level fipronil resistance in this strain. A PCR-based diagnostic assay, capable of screening individual fleas for this mutation, was developed and used to survey a range of fleas collected at random from veterinary clinics in the UK and USA. The A302S mutation was present at a high frequency in these domestic pet populations.

2004 Society of Chemical Industry.
Additional References
RELATED GEPHE
Related Genes
No matches found.
Related Haplotypes
No matches found.
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