GEPHE SUMMARY
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Gephebase Gene
Entry Status
Published
GepheID
GP00002089
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Ipomoea nil - bright blue flowers with colored stems and dark-brown seeds
Trait State in Taxon B
Ipomoea nil - recessive c-1 mutant - white flowers with red stems and colored seeds
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
Japanese morning glory
Synonyms
Convolvulus nil; Pharbitis nil; Japanese morning glory; qian niu; Convolvulus nil L., 1762; Ipomoea nil (L.) Roth, 1797; Pharbitis nil (L.) Choisy, 1834
Rank
species
Lineage
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ina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea
Parent
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
Japanese morning glory
Synonyms
Convolvulus nil; Pharbitis nil; Japanese morning glory; qian niu; Convolvulus nil L., 1762; Ipomoea nil (L.) Roth, 1797; Pharbitis nil (L.) Choisy, 1834
Rank
species
Lineage
Show more ...
ina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea
Parent
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
myb1
Synonyms
-
String
-
Sequence Similarities
-
GO - Molecular Function
GO - Biological Process
-
GO - Cellular Component
UniProtKB
Ipomoea purpurea
Ipomoea purpurea
Presumptive Null
Molecular Type
Aberration Type
Deletion Size
1-9 bp
Molecular Details of the Mutation
frameshift mutation caused by a 2 bp deletion
Experimental Evidence
Authors
Morita Y; Saitoh M; Hoshino A; Nitasaka E; Iida S
Abstract
The transcriptional regulators for anthocyanin biosynthesis include members of proteins containing an R2R3-MYB domain, a bHLH (basic helix-loop-helix) domain and conserved WD40 repeats (WDRs). Spacial and temporal expression of the structural genes encoding the enzymes for anthocyanin biosynthesis is thought to be determined by combinations of the R2R3-MYB, bHLH and WDR factors and their interactions. While the wild-type Japanese morning glory (Ipomoea nil) exhibits blue flowers with colored stems and dark-brown seeds, the c mutants display white flowers with red stems and colored seeds, and the ca mutants exhibit white flowers with green stems and ivory seeds. Here, we characterize the tissue-specific expression of three MYB genes, three bHLH genes and two WDR genes in I. nil. We also show that the recessive c-1 and ca alleles are frameshift mutations caused by a 2 bp deletion and 7 bp insertions in the genes for the R2R3-MYB and WDR transcriptional regulators designated as InMYB1 and InWDR1, respectively. In addition to defects in flower, stem and seed pigmentations, the ca mutants were found to show reduced trichome formation in seeds but to produce leaf and stem trichomes and root hairs normally. Except for the gene for chalcone synthase E in the ca mutant, all structural genes tested were coordinately reduced in both c-1 and ca mutant flower limbs. However, slight but significant expression of the genes for chalcone synthase D, chalcone isomerase and flavanone 3-hydroxylase in the pathway for flavonol biosynthesis was detectable in c-1 and ca mutants, whereas no such residual expression could be observed in other genes involved in the later anthocyanin biosynthesis pathway. The biological roles of the C-1 and Ca genes in I. nil epidermal traits and their evolutionary implications are also discussed.
Additional References
RELATED GEPHE
Related Genes
Related Haplotypes
No matches found.
EXTERNAL LINKS
COMMENTS
MYB1 is also named R2R3-MYB and is is closely related to the petunia (Petunia hybrida) AN2 gene.
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