GEPHE SUMMARY Print
Gephebase Gene
Entry Status
Published
GepheID
GP00002498
Main curator
Courtier
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Aphis gossypii
Trait State in Taxon B
Aphis gossypii - resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Latin Name
Common Name
cotton aphid
Synonyms
cotton aphid; melon aphid; Aphis gossypii Glover, 1877; Aphis gossyppi
Rank
species
Lineage
Show more ... thropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae; Aphidinae; Aphidini; Aphis; Aphis
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Latin Name
Common Name
cotton aphid
Synonyms
cotton aphid; melon aphid; Aphis gossypii Glover, 1877; Aphis gossyppi
Rank
species
Lineage
Show more ... thropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha; Aphidoidea; Aphididae; Aphidinae; Aphidini; Aphis; Aphis
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Presumptive Null
No
Molecular Type
Aberration Type
SNP
SNP Coding Change
Nonsynonymous
Molecular Details of the Mutation
M918L
Experimental Evidence
Taxon A Taxon B Position
Codon - - -
Amino-acid - - -
Authors
Carletto J; Martin T; Vanlerberghe-Masutti F; Brévault T
Abstract
The polyphagous cotton-melon aphid Aphis gossypii Glover is structured into geographically widespread host races comprising a few clones specialised on Cucurbitaceae, cotton, eggplant or pepper. To assess insecticide resistance among and within host races, leaf disc bioassays were conducted on aphid clones collected from Cucurbitaceae (genotypes C4 and C9), cotton (genotypes Burk and Ivo), eggplant (genotype Auber) and pepper (genotype PsP4). Molecular diagnostic (PCR-RFLP) and enzyme assays were also performed to detect the basic mechanisms underlying insecticide resistance.

All six clones were susceptible to acetamiprid (neonicotinoid) or carbosulfan (carbamate). Conversely, all clones were resistant to dimethoate (organophosphate) (RF = 4.1-38.1) and carried mutation S431F in the acetylcholinesterase gene. Auber, PsP4 and Burk also carried mutation A302S in this gene, which possibly conferred moderate resistance (RF = 3.7-6.8) to profenofos and monocrotophos (organophosphates). Auber and Burk were highly resistant (RF = 41.2 and 473 respectively) to cypermethrin (pyrethroid). This resistance was likely associated with point mutation super-kdr (M918L) in the voltage-gated sodium channel gene (para gene) or metabolic detoxification mediated by esterase and oxidase enzymes.

Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.
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