GEPHE SUMMARY
Gephebase Gene
Entry Status
Published
GepheID
GP00000431
Main curator
Martin
PHENOTYPIC CHANGE
Trait Category
Trait State in Taxon A
Helicoverpa armigera - Bt-Cry1Ac susceptible
Trait State in Taxon B
Helicoverpa armigera - Bt-Cry1Ac resistant
Ancestral State
Taxon A
Taxonomic Status
Taxon A
Common Name
cotton bollworm
Synonyms
Heliothis (Helicoverpa) armigera; Heliothis armigera; cotton bollworm; American bollworm; corn ear worm; scarce bordered straw; tobacco budworm; Helicoverpa armigera (Hubner, 1808)
Rank
species
Lineage
Show more ... da; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Heliothinae; Helicoverpa
NCBI Taxonomy ID
is Taxon A an Infraspecies?
No
Taxon B
Common Name
cotton bollworm
Synonyms
Heliothis (Helicoverpa) armigera; Heliothis armigera; cotton bollworm; American bollworm; corn ear worm; scarce bordered straw; tobacco budworm; Helicoverpa armigera (Hubner, 1808)
Rank
species
Lineage
Show more ... da; Insecta; Dicondylia; Pterygota; Neoptera; Holometabola; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Noctuoidea; Noctuidae; Heliothinae; Helicoverpa
NCBI Taxonomy ID
is Taxon B an Infraspecies?
No
GENOTYPIC CHANGE
Generic Gene Name
ABCA2
Synonyms
-
String
-
Sequence Similarities
-
GO - Biological Process
-
UniProtKB
Helicoverpa armigera
GenebankID or UniProtKB
Presumptive Null
Yes
Molecular Type
Aberration Type
Insertion Size
1-10 kb
Molecular Details of the Mutation
Insertion of a DNA transposon
Experimental Evidence
Authors
Zhao J; Jin L; Yang Y; Wu Y
Abstract
Transgenic cotton expressing Bacillus thuringiensis (Bt) toxins has been widely adopted to control some key lepidopteran pests including the bollworm Helicoverpa armigera. Evolution of resistance to Bt cotton by target pests is a major threat to the continued success of Bt cotton. Previous results revealed 3 null alleles (r1-r3) of a cadherin gene (Ha_BtR) conferring Cry1Ac resistance in H. armigera. An F(1) screen of 123 single-pair families was conducted between a Cry1Ac-resistant strain (the SCD-r1 strain, homozygous for the r1 allele of Ha_BtR) and field-derived insects from Jiangpu population (Jiangsu province, China) in 2008. Five new null alleles of Ha_BtR (r4-r8) were identified in six candidate single-pair families. These null alleles were created through either an insertion or a point mutation. Interestingly, intact alleles of Ha_BtR were found in two field-derived insects from another two candidate single-pair families. It suggests that these two field-derived insects may carry novel resistance alleles of Ha_BtR, with missense mutations resulting in a non-functional cadherin protein, or a major dominant mutation at a locus other than cadherin. The resistance allele frequency of Ha_BtR was detected at an appreciable level (0.024) in the Jiangpu population of H. armigera in 2008. Together with previous findings, a total of eight different resistance alleles of Ha_BtR were identified from three Chinese strains of H. armigera. Mutational diversity of Ha_BtR could impair DNA screening for Bt resistance allele frequency in the field, and an F(1) screen should be used routinely for monitoring cadherin-based resistance allele frequencies in H. armigera.

2010 Elsevier Ltd. All rights reserved.
RELATED GEPHE
Related Haplotypes
7
COMMENTS
@TE Parallelism: repeated loss-of-function
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